中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (7): 1039-1044.doi: 10.3969/j.issn.2095-4344.2014.07.010

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

退变和健康椎间盘组织提取细胞外基质诱导背根神经节轴突生长的差异

张  辉1，刘  林2，薛  文2   

  1. 1甘肃省第二人民医院骨科，甘肃省兰州市  730000
    2甘肃省人民医院骨科，甘肃省兰州市  730000
  • 修回日期:2013-12-01 出版日期:2014-02-12 发布日期:2014-02-12
  • 通讯作者: 刘林，主任医师，甘肃省人民医院骨科，甘肃省兰州市 730000
  • 作者简介:张辉，男，1983年生，陕西省镇安县人，汉族，2010年甘肃中医学院毕业，硕士，主治医师，主要从事骨科脊柱脊髓的研究。
  • 基金资助:

    甘肃省自然科学基金资助项目(2010GS04988)

Extracelluar matrix stimulates neurite outgrowth of dorsal root ganglion neurons differently depending on whether extracted from degenerated or normal intervertebral disk

Zhang Hui1, Liu Lin2, Xue Wen2   

  1. 1Department of Orthopedics, Second People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
    2Department of Orthopedics, People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
  • Revised:2013-12-01 Online:2014-02-12 Published:2014-02-12
  • Contact: Liu Lin, Chief physician, Department of Orthopaedics, People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
  • About author:Zhang Hui, Master, Attending physician, Department of Orthopaedics, Second People’s Hospital of Gansu Province, Lanzhou 730000, Gansu Province, China
  • Supported by:

    the Natural Science Foundation of Gansu Province, No. 2010GS04988

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摘要：

背景：研究发现，在退变的椎间盘组织中神经纤维的密度明显高于正常椎间盘，神经末梢和神经肽的出现远远多于正常数量。而正常生理老化的椎间盘并没有这种现象发生。
目的：评估人痛性椎间盘细胞外基质对小鼠背根神经节神经轴突生长所产生的促进作用和神经肽P物质的诱导作用。
方法：获取椎间盘源性下腰痛患者的椎间盘组织(退变组)，以及正常人腰椎间盘组织(正常组)，提取细胞基质，对大鼠背根神经节神经元进行培养，通过形态学观察神经元轴突生长和酶联免疫吸附法检测P物质含量。
结果与结论：退变组神经生长因子浓度显著高于正常组；退变组神经元轴突平均长度明显高于正常组(P < 0.05)，两组加入抗神经生长因子β干预后神经元轴突平均长度均明显短于未干预组；在退变组椎间盘组织培养液中P物质比率明显高于正常组(P < 0.001)。提示人退变椎间盘细胞外基质中高表达的神经生长因子能促进感觉神经轴突的生长以及诱导和疼痛相关神经肽的释放。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 组织工程, 椎间盘, 人痛性椎间盘, 神经生长因子, 生长因子, P物质, 感觉神经, 体外培养, 甘肃省自然科学基金

Abstract:

BACKGROUND: Compared with the normal intervertebral disk, the density of nerve fibers and number of nerve endings and neuropeptides appear to be more in the degenerated intervertebral disk. However, this phenomenon does not occur in the normally aged disk.
OBJECTIVE: To evaluate the axonal growth and induction of a painful neuropeptide and substance P using rat dorsal root ganglion neurons and degenerated human disc cells in vitro.
METHODS: The human intervertebral discs were harvested from patients with discogenic low back pain and normal people. And extracelluar matrix extracted from human degenerative intervertebral discs was cultured with rat dorsal root ganglion neurons. The promotion of axonal growth and induction of substance P of dorsal root ganglion neurons in extracted medium were evaluated through morphology observation and enzyme-linked immunosorbent assay, respectively.
RESULTS AND CONCLUSION: Compared with the normal group, the content of nerve growth factor in the degenerative group was significantly higher and the average length of neuritis was significantly longer in the experimental group (P < 0.05). After intervention with anti-nerve growth factor β, the average length of neuritis became remarkably shorter. The percentage of substance P-immunoreactive cells was significantly higher in the degenerative group compared with the normal group (P < 0.001). Nerve growth factors that highly express in the  extracellular matrix from the degenerative intervertebral dick can promote neurite outgrowth of dorsal root ganglion neurons and induce release of neuropeptides related to pain transmission.



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words: intervertebral disk, intervertebral disk degeneration, nerve growth factor, substance P

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